{"id":751501,"date":"2026-03-21T10:47:42","date_gmt":"2026-03-21T02:47:42","guid":{"rendered":"https:\/\/www.alphaseek.cn\/?p=751501"},"modified":"2026-03-21T10:47:42","modified_gmt":"2026-03-21T02:47:42","slug":"cell-rep%ef%bc%9a%e5%86%9b%e4%ba%8b%e5%8c%bb%e5%ad%a6%e7%a0%94%e7%a9%b6%e9%99%a2%e7%8e%8b%e4%bb%a5%e6%94%bf%e5%9b%a2%e9%98%9f%e6%8f%ad%e7%a4%bavta%e6%98%9f%e5%bd%a2%e8%83%b6%e8%b4%a8%e7%bb%86%e8%83%9e","status":"publish","type":"post","link":"https:\/\/www.alphaseek.cn\/?p=751501","title":{"rendered":"Cell Rep\uff1a\u519b\u4e8b\u533b\u5b66\u7814\u7a76\u9662\u738b\u4ee5\u653f\u56e2\u961f\u63ed\u793aVTA\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u8c03\u63a7GABA\u4f20\u9012\u7684\u6297\u7126\u8651\u6291\u90c1\u673a\u5236"},"content":{"rendered":"
\n

\u6587 | \u8111\u58f0\u5e38\u8c08<\/p>\n<\/blockquote>\n

\u6211\u4eec\u901a\u5e38\u8ba4\u4e3a\u60c5\u7eea\u95ee\u9898\uff08\u6bd4\u5982\u7126\u8651\u3001\u6291\u90c1\uff09\u4e3b\u8981\u548c\u795e\u7ecf\u5143\u6709\u5173\uff0c\u4f46\u5176\u5b9e\u5927\u8111\u91cc\u8fd8\u6709\u4e00\u7c7b\u6570\u91cf\u66f4\u591a\u3001\u957f\u671f\u88ab\u5ffd\u89c6\u7684“\u914d\u89d2”\u7ec6\u80de\uff1a\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u3002\u5b83\u4eec\u8fc7\u53bb\u88ab\u8ba4\u4e3a\u53ea\u662f\u7ed9\u795e\u7ecf\u5143“\u6253\u4e0b\u624b”\u7684\u652f\u6301\u7ec6\u80de\uff0c\u4f46\u73b0\u5728\u79d1\u5b66\u5bb6\u53d1\u73b0\uff0c\u5b83\u4eec\u5176\u5b9e\u80fd\u4e3b\u52a8\u53c2\u4e0e\u8c03\u8282\u5927\u8111\u4fe1\u53f7\uff0c\u5f71\u54cd\u60c5\u7eea\u548c\u884c\u4e3a\u3002<\/span><\/span><\/p>\n

\u57fa\u4e8e\u6b64\uff0c\u519b\u4e8b\u533b\u5b66\u7814\u7a76\u9662\u738b\u4ee5\u653f<\/span>\u56e2\u961f\u5728\u300aCell Reports\u300b<\/span>\u6742\u5fd7\u53d1\u8868\u4e86“Astrocytic modulation of GABA transmission in the VTA limits progression of anxiety- and depressionlike behaviors in adult mice”\u63ed\u793a\u4e86\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u5bf9\u8179\u4fa7\u88ab\u76d6\u533a\uff08VTA\uff09GABA\u4f20\u9012\u7684\u8c03\u63a7\u53ef\u9650\u5236\u6210\u5e74\u5c0f\u9f20\u7126\u8651\u6837\u548c\u6291\u90c1\u6837\u884c\u4e3a\u7684\u53d1\u5c55\u3002<\/span><\/span><\/p>\n

\"Cell<\/span><\/span><\/p>\n

\u519b\u4e8b\u533b\u5b66\u7814\u7a76\u9662\u738b\u4ee5\u653f\u7814\u7a76\u5458\u548c\u674e\u4f73\u59ae\u52a9\u7406\u7814\u7a76\u5458\u4e3a\u8be5\u7814\u7a76\u7684\u5171\u540c\u901a\u8baf<\/a>\u4f5c\u8005\u3002\u519b\u4e8b\u533b\u5b66\u7814\u7a76\u9662\u675c\u519b\u535a\u58eb\u548c\u5201\u534e\u9e4f\u535a\u58eb\u4e3a\u8bba\u6587\u7684\u5171\u540c\u7b2c\u4e00\u4f5c\u8005\u3002<\/span><\/span><\/span><\/span><\/p>\n

\u4f5c\u8005\u53d1\u73b0\uff0c\u5bf9\u538b\u529b\u6709\u53cd\u5e94\u7684VTA\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u53ef\u51cf\u5f31\u5bf9\u591a\u5df4\u80fa\u80fd\u795e\u7ecf\u5143\u7684\u6291\u5236\u4f5c\u7528\uff0c\u4ece\u800c\u7f13\u89e3\u6210\u5e74\u5c0f\u9f20\u7126\u8651\u6837\u548c\u6291\u90c1\u6837\u884c\u4e3a\u7684\u53d1\u5c55\u3002\u5e94\u6fc0\u6e90\u7279\u5f02\u6027\u5730\u5f15\u53d1VTA\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u5185Ca²\u207a\u6c34\u5e73\u5347\u9ad8\u3002\u901a\u8fc7\u5149\u9057\u4f20\u5b66\u6fc0\u6d3bVTA\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\uff0c\u53ef\u7ecf\u7531GABA\u80fd\u4e2d\u95f4\u795e\u7ecf\u5143\u7a81\u89e6\u524d\u7684\u817a\u82f7A1\u53d7\u4f53\u89e3\u9664\u5bf9\u591a\u5df4\u80fa\u80fd\u795e\u7ecf\u5143\u7684\u6291\u5236\uff0c\u4ea7\u751f\u6297\u7126\u8651\u548c\u6297\u6291\u90c1\u6548\u5e94\u3002\u82e5\u901a\u8fc7\u4e0b\u8c03\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u4e2d\u76842\u578b\u808c\u91871,4,5-\u4e09\u78f7\u9178\u53d7\u4f53\uff08IP<\/span><\/span>3<\/span><\/sub><\/span>R2\uff09\u6765\u7834\u574fCa²\u207a\u4fe1\u53f7\u4f20\u5bfc\uff0c\u5219\u4f1a\u589e\u5f3a\u5bf9\u591a\u5df4\u80fa\u80fd\u795e\u7ecf\u5143\u7684\u6291\u5236\u3002\u5728VTA\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u4e2d\u6572\u4f4eIP<\/span><\/span>3<\/span><\/sub><\/span>R2\u6216\u635f\u5bb3\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u80f6\u8d28\u9012\u8d28\u91ca\u653e\u7684\u5c0f\u9f20\uff0c\u8868\u73b0\u51fa\u7126\u8651\u6837\u8868\u578b\u5e76\u5bf9\u8bf1\u53d1\u6291\u90c1\u6837\u884c\u4e3a\u7684\u6162\u6027\u5e94\u6fc0\u66f4\u4e3a\u654f\u611f\u3002\u7efc\u4e0a\u6240\u8ff0\uff0c\u4f5c\u8005\u7684\u7814\u7a76\u63ed\u793a\u4e86VTA\u4e2d\u5b58\u5728\u4e00\u7fa4\u5bf9\u538b\u529b\u6709\u53cd\u5e94\u7684\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\uff0c\u5e76\u5f3a\u8c03\u4e86\u5b83\u4eec\u5728\u9650\u5236\u7126\u8651\u6837\u548c\u6291\u90c1\u6837\u884c\u4e3a\u53d1\u5c55\u4e2d\u7684\u5173\u952e\u4f5c\u7528\u3002<\/span><\/span><\/p>\n

\"Cell<\/span><\/span><\/p>\n

\u56fe\u4e00 \u5e94\u6fc0\u6e90\u8bf1\u5bfc\u4e86VTA\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u4e2dCa²\u207a\u6c34\u5e73\u7684\u5347\u9ad8<\/span><\/span><\/span><\/p>\n

\u5df2\u6709\u7814\u7a76\u8868\u660e\uff0c\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u7684\u6d3b\u52a8\u4e0e\u7ec6\u80de\u5185\u9499\u79bb\u5b50\uff08Ca²\u207a\uff09\u4fe1\u53f7\u5bc6\u5207\u76f8\u5173\u3002\u4e3a\u63a2\u7a76\u8179\u4fa7\u88ab\u76d6\u533a\uff08VTA\uff09\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u5728\u5e94\u5bf9\u538c\u6076\u523a\u6fc0\u65f6\u7684\u6f5c\u5728\u4f5c\u7528\uff0c\u4f5c\u8005\u9996\u5148\u5229\u7528\u5149\u7ea4\u8bb0\u5f55\u7cfb\u7edf\uff0c\u5728\u81ea\u7531\u6d3b\u52a8\u7684\u5c0f\u9f20\u4e2d\u76d1\u6d4b\u4e86VTA\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u5185\u7684Ca²\u207a\u52a8\u6001\u53d8\u5316\u3002<\/span><\/span><\/p>\n

\u591a\u79cd\u5e94\u6fc0\u523a\u6fc0\uff0c\u5305\u62ec<\/span><\/span>\u60ac\u5c3e\u3001\u675f\u7f1a\u5e94\u6fc0\u548c\u7535\u8db3\u5e95\u523a\u6fc0<\/span><\/span>\u5747\u5728\u6ce8\u5c04GCaMP6f\u7684\u5c0f\u9f20\u4e2d\u5f15\u8d77\u663e\u8457\u7684Ca²\u207a\u8367\u5149\u4fe1\u53f7\u5347\u9ad8\uff0c\u800c\u5728\u5bf9\u7167\u5c0f\u9f20\u4e2d\u672a\u68c0\u6d4b\u5230\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u7684Ca²\u207a\u53cd\u5e94\u3002\u6b64\u5916\uff0c\u4e0e\u5bf9\u7167\u5c0f\u9f20\u76f8\u6bd4\uff0cGCaMP6f\u6ce8\u5c04\u5c0f\u9f20\u5728\u5e94\u6fc0\u671f\u95f4Ca²\u207a\u8367\u5149\u77ac\u53d8\u7684\u66f2\u7ebf\u4e0b\u9762\u79ef\u663e\u8457\u589e\u52a0\u3002\u76f8\u6bd4\u4e4b\u4e0b\uff0c\u5956\u8d4f\u523a\u6fc0\uff08\u5305\u62ec\u8517\u7cd6\u6216\u98df\u7269\u6444\u5165\u4ee5\u53ca\u4e0e\u96cc\u6027\u5c0f\u9f20\u7684\u793e\u4f1a\u4e92\u52a8\uff09\u5e76\u672a\u6539\u53d8GCaMP6f\u6ce8\u5c04\u5c0f\u9f20VTA\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u4e2d\u7684Ca²\u207a\u8367\u5149\u4fe1\u53f7\u3002<\/span><\/span><\/p>\n

\u7efc\u4e0a\u6240\u8ff0\uff0c<\/span><\/span>VTA\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u7279\u5f02\u6027\u5730\u88ab\u5e94\u6fc0\u523a\u6fc0\u6fc0\u6d3b\uff0c\u800c\u975e\u5956\u8d4f\u523a\u6fc0\u3002<\/span><\/span><\/p>\n

\"Cell<\/span><\/span><\/p>\n

\u56fe\u4e8c \u5149\u9057\u4f20\u5b66\u6fc0\u6d3bVTA\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u53ef\u589e\u5f3a\u591a\u5df4\u80fa\u80fd\u795e\u7ecf\u5143\u7684\u6d3b\u52a8\uff0c\u5e76\u4ea7\u751f\u6297\u7126\u8651\u548c\u6297\u6291\u90c1\u6548\u5e94<\/span><\/span><\/p>\n

\u7814\u7a76\u4eba\u5458\u53d1\u73b0\uff0c\u6fc0\u6d3bVTA\u7684\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u53ef\u663e\u8457\u589e\u5f3a\u591a\u5df4\u80fa\u80fd\u795e\u7ecf\u5143\u6d3b\u6027\uff0c\u5e76\u4fc3\u8fdbNAc\u4e2d\u591a\u5df4\u80fa\u7684\u91ca\u653e\u3002<\/span><\/span><\/p>\n

\u884c\u4e3a\u5b66\u5b9e\u9a8c\u663e\u793a\uff0c\u8fd9\u79cd\u6fc0\u6d3b\u80fd\u6709\u6548\u7f13\u89e3\u5c0f\u9f20\u7684\u7126\u8651\u6837\u884c\u4e3a\uff08\u5982\u5728<\/span><\/span>\u9ad8\u67b6\u5341\u5b57\u8ff7\u5bab\uff08<\/span><\/span>VisuTrack\uff0c\u4e0a\u6d77\u6b23\u8f6f<\/span><\/a><\/span><\/span><\/span><\/span>\uff09<\/span><\/span>\u4e2d\u66f4\u503e\u5411\u4e8e\u63a2\u7d22\u5f00\u653e\u81c2\uff09\u3001\u51cf\u8f7b\u6291\u90c1\u6837\u8868\u73b0\uff08<\/span><\/span>\u60ac\u5c3e\u5b9e\u9a8c<\/span><\/span>\u4e2d\u4e0d\u52a8\u65f6\u95f4\u51cf\u5c11\uff09\uff0c\u5e76\u8bf1\u5bfc\u660e\u663e\u7684\u5956\u8d4f\u6548\u5e94\uff08\u5c0f\u9f20\u504f\u597d\u4e0e\u5149\u523a\u6fc0\u914d\u5bf9\u7684\u73af\u5883<\/span><\/span>VisuTrack\uff0c\u4e0a\u6d77\u6b23\u8f6f<\/span><\/a><\/span><\/span>\uff09\u3002<\/span><\/span><\/p>\n

\u8fdb\u4e00\u6b65\u673a\u5236\u7814\u7a76\u8868\u660e\uff0c\u5f53\u4f7f\u7528D1\u591a\u5df4\u80fa\u53d7\u4f53\u62ee\u6297\u5242SCH23390\u963b\u65ad\u591a\u5df4\u80fa\u4fe1\u53f7\u540e\uff0c\u4e0a\u8ff0\u6297\u7126\u8651\u3001\u6297\u6291\u90c1\u53ca\u5956\u8d4f\u4f5c\u7528\u5747\u88ab\u5b8c\u5168\u6d88\u9664\u3002<\/span><\/span><\/p>\n

\u8fd9\u4e9b\u7ed3\u679c\u8868\u660e\uff0c<\/span><\/span>VTA\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u901a\u8fc7\u8c03\u63a7D1\u53d7\u4f53\u4f9d\u8d56\u7684\u591a\u5df4\u80fa\u901a\u8def\uff0c\u53d1\u6325\u60c5\u7eea\u8c03\u8282\u548c\u6b63\u5411\u5f3a\u5316\u4f5c\u7528\uff0c\u63ed\u793a\u4e86\u80f6\u8d28\u7ec6\u80de\u5728\u60c5\u611f\u884c\u4e3a\u4e2d\u7684\u5173\u952e\u89d2\u8272<\/span><\/span>\u3002<\/span><\/span><\/p>\n

\"Cell<\/span><\/span><\/p>\n

\u56fe\u4e09 \u7834\u574fVTA\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u7684\u529f\u80fd\u4f1a\u589e\u52a0\u5e94\u6fc0\u6240\u81f4\u7684\u6291\u90c1\u6613\u611f\u6027<\/span><\/span><\/p>\n

\u4e3a\u63a2\u7a76VTA\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u7684\u9499\u4fe1\u53f7\u662f\u5426\u5f71\u54cd\u60c5\u7eea\u884c\u4e3a\uff0c\u7814\u7a76\u4eba\u5458\u901a\u8fc7\u4e0b\u8c03\u5176\u5173\u952e\u9499\u901a\u9053\u86cb\u767dIP3R2\uff08\u8868\u8fbeip3r2 shRNA\uff09\u6765\u5e72\u6270Ca²\u207a\u4f9d\u8d56\u7684\u6d3b\u6027\u3002\u7ed3\u679c\u663e\u793a\uff0c\u5728\u6b63\u5e38\u72b6\u6001\u4e0b\uff0c\u8fd9\u4e9b\u5c0f\u9f20\u5e76\u672a\u8868\u73b0\u51fa\u660e\u663e\u6291\u90c1\u6837\u884c\u4e3a\uff0c\u4f46\u5728<\/span><\/span>\u65f7\u573a\u5b9e\u9a8c\u548c\u9ad8\u67b6\u5341\u5b57\u8ff7\u5bab<\/span><\/span>\u4e2d\u63a2\u7d22\u4e2d\u5fc3\u533a\u6216\u5f00\u653e\u81c2\u7684\u65f6\u95f4\u663e\u8457\u51cf\u5c11\uff0c\u63d0\u793a\u51fa\u73b0\u7126\u8651\u6837\u8868\u73b0\u3002\u66f4\u91cd\u8981\u7684\u662f\uff0c\u5728\u7ecf\u538610\u5929<\/span><\/span>\u6162\u6027\u6e29\u548c\u4e0d\u53ef\u9884\u77e5\u5e94\u6fc0<\/span><\/span>\u540e\uff0cIP3R2\u88ab\u6291\u5236\u7684\u5c0f\u9f20\u4e0d\u4ec5\u7126\u8651\u52a0\u91cd\uff0c\u8fd8\u51fa\u73b0\u4e86\u5178\u578b\u7684\u6291\u90c1\u6837\u884c\u4e3a\u5982<\/span><\/span>\u7cd6\u6c34\u504f\u597d<\/span><\/span>\u4e0b\u964d\u3001<\/span><\/span>\u60ac\u5c3e\u548c\u5f3a\u8feb\u6e38\u6cf3<\/span><\/span>\u4e2d\u4e0d\u52a8\u65f6\u95f4\u589e\u52a0\u3002<\/span><\/span><\/p>\n

\u7c7b\u4f3c\u5730\uff0c\u5f53\u7814\u7a76\u4eba\u5458\u7528\u5149\u9057\u4f20\u5de5\u5177\uff08Opto vTrap\uff09\u7279\u5f02\u6027\u6291\u5236VTA\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u91ca\u653e\u80f6\u8d28\u9012\u8d28\u65f6\uff0c\u5c0f\u9f20\u5728\u5e94\u6fc0\u6761\u4ef6\u4e0b\u540c\u6837\u8868\u73b0\u51fa\u66f4\u5f3a\u7684\u7126\u8651\u548c\u6291\u90c1\u6837\u53cd\u5e94\uff0c\u5305\u62ec\u6d3b\u52a8\u51cf\u5c11\u3001\u56de\u907f\u5f00\u653e\u7a7a\u95f4\u3001\u5feb\u611f\u7f3a\u5931\u548c\u884c\u4e3a\u7edd\u671b\u3002<\/span><\/span><\/p>\n

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